Urea unfolding of peptide helices as a model for interpreting protein unfolding.
نویسندگان
چکیده
منابع مشابه
Mechanical unfolding pathway of a model β-peptide foldamer.
Foldamers constructed from oligomers of β-peptides form stable secondary helix structures already for small chain lengths, which makes them ideal candidates for the investigation of the (un)folding of polypeptides. Here, the results of molecular simulations of the mechanical unfolding of a β-heptapeptide in methanol solvent revealing the detailed unfolding pathway are reported. The unfolding pr...
متن کاملProtein Unfolding and the Diffusion Collision Model
In the diffusion-collision model, the unfolding or backward rates are given by the likelihood of secondary structural cluster dissociation. In this work, we introduce a backward rate calculation modeled from a Kramers-type thermal tunneling through a barrier, which represents the free energy potential well for buried hydrophobic residues. Our results are in good agreement with currently accepte...
متن کاملTemperature dependent protein unfolding
Protein structure prediction is a central problem in current biophysics. Understanding the energy landscape roughness would shed light on the physics behind protein folding. The theory for extracting energy landscape roughness from temperature dependent unfolding measurements is reviewed. Improvements facilitating temperature dependent single molecule protein unfolding experiments are described...
متن کاملProtein unfolding by mitochondria
Protein unfolding is a key step in the import of some proteins into mitochondria and chloroplasts and in the degradation of regulatory proteins by ATP-dependent proteases. In contrast to protein folding, the reverse process has remained largely uninvestigated until now. This review discusses recent discoveries on the mechanism of protein unfolding during translocation into mitochondria. The mit...
متن کاملProtein unfolding: rigidity lost.
We relate the unfolding of a protein to its loss of structural stability or rigidity. Rigidity and flexibility are well defined concepts in mathematics and physics, with a body of theorems and algorithms that have been applied successfully to materials, allowing the constraints in a network to be related to its deformability. Here we simulate the weakening or dilution of the noncovalent bonds d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.1.185